Immunogenicity, safety, and protective efficacy of an inactivated SARS-associated coronavirus vaccine in rhesus monkeys
Identifieur interne : 004798 ( Main/Exploration ); précédent : 004797; suivant : 004799Immunogenicity, safety, and protective efficacy of an inactivated SARS-associated coronavirus vaccine in rhesus monkeys
Auteurs : Jun Zhou [République populaire de Chine] ; Wei Wang [République populaire de Chine] ; Qiong Zhong [République populaire de Chine] ; Wei Hou [République populaire de Chine] ; Zhanqiu Yang [République populaire de Chine] ; Shu-Yuan Xiao [États-Unis] ; Runqing Zhu [République populaire de Chine] ; Zhijiao Tang [République populaire de Chine] ; Yong Wang [République populaire de Chine] ; Qiaoyang Xian [République populaire de Chine] ; Hongbing Tang [République populaire de Chine] ; Li Wen [République populaire de Chine]Source :
- Vaccine [ 0264-410X ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Cytokines (biosynthèse), Femelle, Immunité muqueuse (immunologie), Immunoglobuline A (analyse), Immunoglobuline A (biosynthèse), Immunoglobuline G (analyse), Immunoglobuline G (biosynthèse), Macaca mulatta, Mâle, RT-PCR, Radiographie thoracique, Syndrome respiratoire aigu sévère (imagerie diagnostique), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Test ELISA, Tests de neutralisation, Vaccins antiviraux (effets indésirables), Vaccins antiviraux (immunologie), Vaccins inactivés (effets indésirables), Vaccins inactivés (immunologie), Virus du SRAS (immunologie).
- MESH :
- analyse : Immunoglobuline A, Immunoglobuline G.
- biosynthèse : Cytokines, Immunoglobuline A, Immunoglobuline G.
- effets indésirables : Vaccins antiviraux, Vaccins inactivés.
- imagerie diagnostique : Syndrome respiratoire aigu sévère.
- immunologie : Immunité muqueuse, Syndrome respiratoire aigu sévère, Vaccins antiviraux, Vaccins inactivés, Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Femelle, Macaca mulatta, Mâle, RT-PCR, Radiographie thoracique, Test ELISA, Tests de neutralisation.
English descriptors
- KwdEn :
- Animals, Cytokines (biosynthesis), Enzyme-Linked Immunosorbent Assay, Female, Immunity, Mucosal (immunology), Immunoglobulin A (analysis), Immunoglobulin A (biosynthesis), Immunoglobulin G (analysis), Immunoglobulin G (biosynthesis), Macaca mulatta, Male, Neutralization Tests, Radiography, Thoracic, Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (immunology), Severe Acute Respiratory Syndrome (diagnostic imaging), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Vaccines, Inactivated (adverse effects), Vaccines, Inactivated (immunology), Viral Vaccines (adverse effects), Viral Vaccines (immunology).
- MESH :
- chemical , adverse effects : Vaccines, Inactivated, Viral Vaccines.
- chemical , analysis : Immunoglobulin A, Immunoglobulin G.
- chemical , biosynthesis : Cytokines, Immunoglobulin A, Immunoglobulin G.
- diagnostic imaging : Severe Acute Respiratory Syndrome.
- immunology : Immunity, Mucosal, SARS Virus, Severe Acute Respiratory Syndrome, Vaccines, Inactivated, Viral Vaccines.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Enzyme-Linked Immunosorbent Assay, Female, Macaca mulatta, Male, Neutralization Tests, Radiography, Thoracic, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
An inactivated vaccine for severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) was evaluated in rhesus monkeys. The monkeys were inoculated intramuscularly (i.m.) with 0.5, 5, 50, or 5000 μg of vaccine, or PBS as control, and boosted on day 7. After 3 weeks, they were challenged with the NS-1 strain of SARS-CoV. The humoral and mucosal immune responses, clinical signs, chemical indices and viremia were monitored following the immunization and challenge. The control animals who received PBS developed atypical SAR-CoV infection after viral challenge, according to clinical, virological and pathological findings. No systematic side effects were observed in vaccinated animals post-immunization, even in at the high dose of 5000 μg. The 50 μg dosage of vaccine elicited SARS-CoV specific immune responses against viral infection as compared to the partial immunity elicited by 0.5 and 5 μg doses. The results show that this inactivated vaccine can induce effective concomitant humoral and mucosal immunity against SARS-CoV infection, is safe in monkeys, and the vaccine maybe a good candidate for clinical trials.
Url:
DOI: 10.1016/j.vaccine.2004.11.075
PubMed: 15837221
PubMed Central: 7115379
Affiliations:
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Le document en format XML
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wicri:level="1"><nlm:aff id="aff1">Institute of Virology, Key Laboratory of Virology (Wuhan University) Ministry of Education, Wuhan 430071, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Virology, Key Laboratory of Virology (Wuhan University) Ministry of Education, Wuhan 430071</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Zhong, Qiong" sort="Zhong, Qiong" uniqKey="Zhong Q" first="Qiong" last="Zhong">Qiong Zhong</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Virology, Key Laboratory of Virology (Wuhan University) Ministry of Education, Wuhan 430071, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Virology, Key Laboratory of Virology (Wuhan University) Ministry of Education, Wuhan 430071</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Hou, Wei" sort="Hou, Wei" uniqKey="Hou W" first="Wei" last="Hou">Wei Hou</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Virology, Key Laboratory of Virology (Wuhan University) Ministry of Education, Wuhan 430071, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Virology, Key Laboratory of Virology (Wuhan University) Ministry of Education, Wuhan 430071</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Yang, Zhanqiu" sort="Yang, Zhanqiu" uniqKey="Yang Z" first="Zhanqiu" last="Yang">Zhanqiu Yang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Virology, Key Laboratory of Virology (Wuhan University) Ministry of Education, Wuhan 430071, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Virology, Key Laboratory of Virology (Wuhan University) Ministry of Education, Wuhan 430071</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Xiao, Shu Yuan" sort="Xiao, Shu Yuan" uniqKey="Xiao S" first="Shu-Yuan" last="Xiao">Shu-Yuan Xiao</name><affiliation wicri:level="2"><nlm:aff id="aff4">Departments of Pathology and Internal Medicine, University of Texas Medical Branch, Galveston, TX, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Departments of Pathology and Internal Medicine, University of Texas Medical Branch, Galveston, TX</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Zhu, Runqing" sort="Zhu, Runqing" uniqKey="Zhu R" first="Runqing" last="Zhu">Runqing Zhu</name><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Pathology, Wuhan University School of Medicine, Wuhan 430071, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathology, Wuhan University School of Medicine, Wuhan 430071</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Tang, Zhijiao" sort="Tang, Zhijiao" uniqKey="Tang Z" first="Zhijiao" last="Tang">Zhijiao Tang</name><affiliation wicri:level="1"><nlm:aff id="aff3">Experimental Animal Center, Wuhan University School of Medicine, Wuhan 430071, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Animal Center, Wuhan University School of Medicine, Wuhan 430071</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Wang, Yong" sort="Wang, Yong" uniqKey="Wang Y" first="Yong" last="Wang">Yong Wang</name><affiliation wicri:level="1"><nlm:aff id="aff3">Experimental Animal Center, Wuhan University School of Medicine, Wuhan 430071, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Animal Center, Wuhan University School of Medicine, Wuhan 430071</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Xian, Qiaoyang" sort="Xian, Qiaoyang" uniqKey="Xian Q" first="Qiaoyang" last="Xian">Qiaoyang Xian</name><affiliation wicri:level="1"><nlm:aff id="aff3">Experimental Animal Center, Wuhan University School of Medicine, Wuhan 430071, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Animal Center, Wuhan University School of Medicine, Wuhan 430071</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Tang, Hongbing" sort="Tang, Hongbing" uniqKey="Tang H" first="Hongbing" last="Tang">Hongbing Tang</name><affiliation wicri:level="1"><nlm:aff id="aff3">Experimental Animal Center, Wuhan University School of Medicine, Wuhan 430071, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Animal Center, Wuhan University School of Medicine, Wuhan 430071</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Wen, Li" sort="Wen, Li" uniqKey="Wen L" first="Li" last="Wen">Li Wen</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Virology, Key Laboratory of Virology (Wuhan University) Ministry of Education, Wuhan 430071, PR China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Virology, Key Laboratory of Virology (Wuhan University) Ministry of Education, Wuhan 430071</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><idno type="eISSN">1873-2518</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cytokines (biosynthesis)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Immunity, Mucosal (immunology)</term><term>Immunoglobulin A (analysis)</term><term>Immunoglobulin A (biosynthesis)</term><term>Immunoglobulin G (analysis)</term><term>Immunoglobulin G (biosynthesis)</term><term>Macaca mulatta</term><term>Male</term><term>Neutralization Tests</term><term>Radiography, Thoracic</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (diagnostic imaging)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Vaccines, Inactivated (adverse effects)</term><term>Vaccines, Inactivated (immunology)</term><term>Viral Vaccines (adverse effects)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cytokines (biosynthèse)</term><term>Femelle</term><term>Immunité muqueuse (immunologie)</term><term>Immunoglobuline A (analyse)</term><term>Immunoglobuline A (biosynthèse)</term><term>Immunoglobuline G (analyse)</term><term>Immunoglobuline G (biosynthèse)</term><term>Macaca mulatta</term><term>Mâle</term><term>RT-PCR</term><term>Radiographie thoracique</term><term>Syndrome respiratoire aigu sévère (imagerie diagnostique)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Test ELISA</term><term>Tests de neutralisation</term><term>Vaccins antiviraux (effets indésirables)</term><term>Vaccins antiviraux (immunologie)</term><term>Vaccins inactivés (effets indésirables)</term><term>Vaccins inactivés (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="adverse effects" xml:lang="en"><term>Vaccines, Inactivated</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Immunoglobulin A</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Cytokines</term><term>Immunoglobulin A</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Immunoglobuline A</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Cytokines</term><term>Immunoglobuline A</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" qualifier="diagnostic imaging" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="effets indésirables" xml:lang="fr"><term>Vaccins antiviraux</term><term>Vaccins inactivés</term></keywords><keywords scheme="MESH" qualifier="imagerie diagnostique" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Immunité muqueuse</term><term>Syndrome respiratoire aigu sévère</term><term>Vaccins antiviraux</term><term>Vaccins inactivés</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Immunity, Mucosal</term><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term><term>Vaccines, Inactivated</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Macaca mulatta</term><term>Male</term><term>Neutralization Tests</term><term>Radiography, Thoracic</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Macaca mulatta</term><term>Mâle</term><term>RT-PCR</term><term>Radiographie thoracique</term><term>Test ELISA</term><term>Tests de neutralisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>An inactivated vaccine for severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) was evaluated in rhesus monkeys. The monkeys were inoculated intramuscularly (i.m.) with 0.5, 5, 50, or 5000 μg of vaccine, or PBS as control, and boosted on day 7. After 3 weeks, they were challenged with the NS-1 strain of SARS-CoV. The humoral and mucosal immune responses, clinical signs, chemical indices and viremia were monitored following the immunization and challenge. The control animals who received PBS developed atypical SAR-CoV infection after viral challenge, according to clinical, virological and pathological findings. No systematic side effects were observed in vaccinated animals post-immunization, even in at the high dose of 5000 μg. The 50 μg dosage of vaccine elicited SARS-CoV specific immune responses against viral infection as compared to the partial immunity elicited by 0.5 and 5 μg doses. 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Scott</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Hubei</li><li>Texas</li></region><settlement><li>Wuhan</li></settlement></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Zhou, Jun" sort="Zhou, Jun" uniqKey="Zhou J" first="Jun" last="Zhou">Jun Zhou</name></region><name sortKey="Hou, Wei" sort="Hou, Wei" uniqKey="Hou W" first="Wei" last="Hou">Wei Hou</name><name sortKey="Tang, Hongbing" sort="Tang, Hongbing" uniqKey="Tang H" first="Hongbing" last="Tang">Hongbing Tang</name><name sortKey="Tang, Zhijiao" sort="Tang, Zhijiao" uniqKey="Tang Z" first="Zhijiao" last="Tang">Zhijiao Tang</name><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name><name sortKey="Wang, Yong" sort="Wang, Yong" uniqKey="Wang Y" first="Yong" last="Wang">Yong Wang</name><name sortKey="Wen, Li" sort="Wen, Li" uniqKey="Wen L" first="Li" last="Wen">Li Wen</name><name sortKey="Xian, Qiaoyang" sort="Xian, Qiaoyang" uniqKey="Xian Q" first="Qiaoyang" last="Xian">Qiaoyang Xian</name><name sortKey="Yang, Zhanqiu" sort="Yang, Zhanqiu" uniqKey="Yang Z" first="Zhanqiu" last="Yang">Zhanqiu Yang</name><name sortKey="Zhong, Qiong" sort="Zhong, Qiong" uniqKey="Zhong Q" first="Qiong" last="Zhong">Qiong Zhong</name><name sortKey="Zhu, Runqing" sort="Zhu, Runqing" uniqKey="Zhu R" first="Runqing" last="Zhu">Runqing Zhu</name></country><country name="États-Unis"><region name="Texas"><name sortKey="Xiao, Shu Yuan" sort="Xiao, Shu Yuan" uniqKey="Xiao S" first="Shu-Yuan" last="Xiao">Shu-Yuan Xiao</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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